IR quartz is a great choice for buckets for UV VIS measurements. The transmission range is 220-3,500 nm, so you get some UV, but you also get a good range in the IR. Not all bucket material will work for every experiment, so some basic education is required. Using this page as a guide, we will walk you through the important factors to consider when buying a UV VIS bucket. These buckets are the ultimate balance for someone looking for a high-quality UV quartz tamper.
The distance traveled by the light through the sample is known as path length. The length between the inner walls of a UV fish bucket where light passes is known as the path of light. The internal distance between the front and rear windows of a conventional spectrophotometer box is the path of the light or the length of the path. Usually a UV fish bucket is chosen with exact dimensions, such as a path length of 10 mm.
If a bucket shows smaller scratches or cracks in the polished field, it should not be used for additional measurements because the result is falsified or the bucket in the spectrophotometer may break. Any resin material used to repair or glue the bucket would compromise the quality of the measurement. For best results, reference and sample measurements should be carried out with the same type of container.
Let’s start with a fundamental understanding of their material needs and then compare and contrast different types of buckets. The length of the optical path of the bucket is determined based on the size of the absorption or transmission of a chemical sample of low or high concentration. We will continue to discuss solutions for samples to be diluted. The length of the optical pad is determined by the side of the double pad length bucket placed on the spectrometer.
The most common type of bucket is square, with external dimensions of 12.5 x 12.5 mm. This format is suitable for sample volumes from the micro-litre range (micro-ultramicro buckets) to the milliliter range. The standard path length of a bucket measures 10 mm; however, buckets are also available that provide a shorter light path through the sample. In addition, buckets differ in terms of their material, their height and the size of their measuring window. The most common type of bucket is square, with external dimensions of 12.5×12.5 mm. This size is suitable for sample volumes from the microlitre (sub-micro buckets) tosemi-micro to the milliliter range or even larger.
This makes them an eco-friendly option that can also save you money by eliminating the ongoing purchase of disposable plastic buckets. For solutions with absorption coefficients that are too high for a standard path length bucket, we have buckets with trajectory lengths as short as 1 mm. You can achieve a tenfold reduction in absorption by simply replacing buckets, instead of wasting time and solvent diluting your solutions. Below is a list of buckets ranging from 10mm buckets from the bottom, lids, way lengths, inner widths, volumes and the number of transparent walls or other shapes available for immediate orders.
If you want to choose one, you need to define the specific use and equipment. For example, plastic may not be efficient for UV range experiments, but it is a cost-effective alternative to all visible light studies. UV quartz veins are designed to contain samples for spectroscopic experiments. We offer both quartz semimers with two polished sides for absorption spectroscopy and quarter-semimers with four polished sides for fluorescence spectroscopy. For measurement best practices, the bin should remain on the bin holder between measurements. If it is removed, it should be ensured that the bucket is always placed in the same direction on the bucket holder, i.e. with the label to the light source.
In general, quartz and glass buckets have greater transmission and accuracy of spectroscopy measurements, and these buckets can be reused much more often. However, the plastic bucket is cheap and easy to use, without the need to clean and prevent cross-contamination, making it an excellent choice for proteins, DNA and RNA and aqueous solutions. The buckets are made of glass, plastic or quartz of optical quality. Plastic buckets have the advantage of being less expensive and disposable and are often used in rapid spectroscopic tests.
If the bucket is not properly veiled, the absorption sensitivity to the measurement may be reduced. The selection of the equipment requires requirements in the tray, as it must be compatible with the device. This mainly relates to the external dimensions of the bucket, because it must fit into the shaft of the bucket, but also the height Cuvettes of the measuring windows is crucial. These must perfectly match the path of light traveling through the instrument. This consideration is particularly relevant for buckets that are designed to measure small volumes and therefore have very small measuring windows. It is expected to use only quartz veins for UV absorption measurements.